How much DNA do I need to provide?
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How much DNA do I need to provide?
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To perform a DArT assay, we require you to send us about 20-30uL of an aqueous solution of DNA at 50 to 100 ng per µl, not more.
Important
Excessive concentration is detrimental to the assay. If your DNA is concentrated please dilute it.
The assay itself uses 2µl of recommended concentration DNA, however we need a larger volume to allow for any problems during shipment, as well as to be able to perform Quality Control and Method Tests (for new organisms).
We have successfully processed samples with much lower DNA concentration than recommended, including DNA extracted from museum and herbarium specimens often over 100 years old, but the best data quality is assured by providing samples in the recommended range.
Our assays generally tolerate up to 3-4 fold variation in DNA concentration within a plate. Please contact us if you have any questions regarding the quality and/or quantity of your sample’s DNA
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