How do I check that my DNA is suitable for DArT services?

The following method for preparing your DNA will help you to get the best quality data from the samples, in less time and with less wastage. 

To control the DNA quality of the samples:

• Incubate 1-2uL of DNA in restriction enzyme buffer at 37°C for 2 hours 
• Resolve the DNA on a 0.8% agarose gel in 1x TAE buffer 
• Inspect the gel image for your samples.

Good quality DNA gives a high molecular weight band on the gel image. This protocol is a mock digest to detect the presence of buffer-activated (usually Mg dependent) nucleases. No restriction enzyme is used. To make sure the DNA is of sufficient quality for DArT genotyping, you can also send us the image of the DNA quality control you already have, for us to ensure the DNA is of sufficient quality for DArT genotyping.